Rail union RMT will take strike action on 14 train operating companies on November 3 and 5 after no new offer on pay jobs and working conditions.
Strike action will coincide with RMT members on Network Rail, London Underground and Overground who are also walking out.
Despite repeated negotiations, the Rail Delivery Group has failed to offer RMT members any offer on pay, jobs and conditions.
RMT general secretary Mick Lynch said: “Throughout this whole dispute, the Rail Delivery Group has been completely unreasonable by not offering our members any deal on pay, conditions and job security.
“Some of our members on the train operating companies are some of the lowest paid on the railways.
“This stands in stark contrast to rail operating company bosses making millions of pounds in profit.
“We remain open to meaningful talks, but we are steadfast in our industrial campaign to see a negotiated settlement for all our members in this dispute.”